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KMID : 1145020190360040220
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Journal of Acupuncture Research
2019 Volume.36 No. 4 p.220 ~ p.229
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The Effects of Bee Venom Pharmacopuncture on Middle Cerebral Artery Occlusion Ischemic Cerebral Damage in Mice
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Lee Ji-In
Song Ho-Sueb
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Abstract
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Background: The therapeutic potential of Bee Venom Pharmacopuncture (BVP) on acute ischemic cerebral infraction was determined in mice in vivo and in vitro.
Methods: Analysis of acute ischemic cerebral infraction was performed using 7 week old male ICR mice (n = 20) and microglial BV-2 cells. Bee venom (5 μg/kg) was injected into the caudal vein of middle cerebral artery occlusion (MCAo) mice (1 hour after reperfusion, 3 hours after MCAo probe insertion), and also used to treat LPS-stimulated microglial BV-2 cells (1, 2, 5 μg/mL). Markers of inflammation were monitored.
Results: NO declined statistically significantly in BVP treated MCAo mice compared to the untreated MCAo group (p < 0.05). Compared to the MCAo group, the BVP-treated MCAo group showed a decreased production volume of malondialdehyde, but an increased glutathione/oxidized glutathione ratio. Compared to the untreated MCAo group, the BVP treated MCAo group showed a statistically significant decline in TNF and IL-1β levels (p < 0.05). BVP inhibited the levels of p65, p50, p-IκB-α, and levels of p-ERK1/2, p-JNK2, p-P38 declined.
Conclusion: BVP is effective at dampening the inflammatory response in vivo and in vitro and may supplement rt-PA treatment.
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KEYWORD
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bee venom, ischemia, middle cerebral artery occlusion, NF-kB, pharmacopuncture, stroke
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